Liquid platelet-rich fibrin promotes the regenerative potential of human periodontal ligament cells

Abstract

Objective: To compare the biological effect of PRP and liquid-PRF on human periodontal ligament cells (hPDLCs) in vitro.

Methods: The liquid-PRF was processed with centrifugation at 700 g for 3 min, and PRP was processed according to Curasan's protocol. Migration and proliferation assay were performed by a scratch/Transwell assay and a CCK-8 assay, respectively. To investigate hPDLC differentiation, alkaline phosphatase (ALP) assay, Alizarin Red S staining, and gene expression level detection of Runx2, Col1a1, and OCN were conducted. Furthermore, cells cultured with lipopolysaccharide (LPS) to induce an inflammation condition were utilized to investigate the impact of liquid-PRF on inflammatory resolution.

Results: Either PRP or liquid-PRF can promote proliferation, migration of hPDLCs, and osteogenic differentiation of hPDLCs. It was noteworthy that liquid-PRF demonstrated a significantly higher ability to promote the biological differentiation and mineralization of hPDLCs compared with PRP. Lastly, when hPDLCs were incubated with LPS, cells cultured with liquid-PRF showed significantly lower mRNA expression levels of inflammatory genes.

Conclusions: Liquid-PRF notably promoted hPDLC activity and attenuated the inflammatory state induced by LPS.

Keywords: Platelet-rich plasma; hPDLCs; liquid platelet-rich fibrin; osteogenic differentiation.