Prediction of Ubiquitin Ligase Nrdp1-Associated Proteins in Glioma Database

Cell Biochem Biophys. 2020 Sep;78(3):301-308. doi: 10.1007/s12013-020-00926-1. Epub 2020 Jun 19.

Abstract

The ubiquitin proteasome pathway is conserved from yeast to mammals and is necessary for the targeted degradation of most short-lived proteins in eukaryotic cells. Its protein substrates include cell cycle regulatory proteins and proteins that are not properly folded in the endoplasmic reticulum. Owing to the ubiquity of its protein substrates, ubiquitination regulates a variety of cellular activities, including cell proliferation, apoptosis, autophagy, endocytosis, DNA damage repair, and immune response. With new genomic data continuously being obtained, ubiquitination through genomic data analysis will be an effective method. We obtained 83 overlapping genes from four glioma databases, which differed from ubiquitin ligase Nrdp1 expression, including 36 downregulated and 47 upregulated genes. The KEGG pathways, molecular functions, cellular components, and biological processes potentially associated with Nrdp1 were obtained using GSEA and Cytoscape. In human gliomas, differences in the expression of Nrdp1 were identified between nontumor brain tissue and different glioma tissues, but no difference in expression was found between low‑grade glioma (LGG) and anaplastic glioma (AG). In survival analysis, we found no significant association between Nrdp1 expression level and patient prognosis.

Keywords: Bioinformatics; GSEA (gene set enrichment analysis); Genomics; Glioma; Nrdp1 (neuregulin receptor degradation protein-1); Ubiquitination.

MeSH terms

  • Brain Neoplasms / genetics*
  • Computational Biology
  • DNA Damage
  • DNA Repair
  • Databases, Protein*
  • Gene Expression Regulation, Neoplastic*
  • Glioma / genetics*
  • Humans
  • Immune System
  • Prognosis
  • Ubiquitin-Protein Ligases / genetics*

Substances

  • RNF41 protein, human
  • Ubiquitin-Protein Ligases