Impact of acute inflammation on Band 3 protein anion exchange capability in human erythrocytes

Arch Physiol Biochem. 2022 Oct;128(5):1242-1248. doi: 10.1080/13813455.2020.1764048. Epub 2020 May 13.

Abstract

Objective: The impact of acute inflammation, revealed by C-reactive protein (CRP) plasma levels, has been studied on the erythrocytes anion exchanger Band 3 protein.Methods: Anion exchange capability through Band 3 protein, lipid peroxidation, -SH membrane groups and intracellular GSH levels have been measured on erythrocytes from patients with CRP >8 mg/L.Results: Under acute inflammation, a significant increase in anion exchange capability, increased lipid peroxidation, decreased-SH groups and GSH content were observed. Serum CRP levels recovery (after one week) was associated to -SH groups and GSH recovery, but not to anion exchange capability restoration. After 2 months, a total recovery of all parameters was observed.Conclusion: Band 3 protein anion exchange capability is affected by acute inflammation; the accelerated rate of anion exchange may be mainly due to lipid peroxidation, rather than to -SH groups oxidation; erythrocytes renewal could be needed to have a total recover of their function.

Keywords: Acute inflammation; Band 3 protein; C-reactive protein; SO4= uptake; anion exchange.

MeSH terms

  • Anion Exchange Protein 1, Erythrocyte* / metabolism
  • Anions / metabolism
  • C-Reactive Protein* / metabolism
  • Erythrocytes / metabolism
  • Humans
  • Inflammation / metabolism
  • Oxidative Stress

Substances

  • Anion Exchange Protein 1, Erythrocyte
  • Anions
  • C-Reactive Protein