Nuclear magnetic resonance analysis of female and male pre-hatching embryo metabolites at the embryo-maternal interface

M Muñoz; J Gatien; P Salvetti; D Martín-González; S Carrocera; E Gómez

doi:10.1007/s11306-020-01672-4

Nuclear magnetic resonance analysis of female and male pre-hatching embryo metabolites at the embryo-maternal interface

Metabolomics. 2020 Apr 8;16(4):47. doi: 10.1007/s11306-020-01672-4.

Authors

M Muñoz¹, J Gatien², P Salvetti², D Martín-González³, S Carrocera³, E Gómez³

Affiliations

¹ Centro de Biotecnología Animal - SERIDA, Camino de Rioseco, 1225, Gijón, Spain. mmunoz@serida.org.
² ALLICE, Experimental Facilities, Le Perroi, 37380, Nouzilly, France.
³ Centro de Biotecnología Animal - SERIDA, Camino de Rioseco, 1225, Gijón, Spain.

PMID: 32270352
DOI: 10.1007/s11306-020-01672-4

Abstract

Introduction: Bovine female and male embryos differentially release metabolites with signalling effects to culture media. However, it is unknown if the embryo-maternal interface (EMI) metabolome is modified by embryonic sex.

Objective: To analyse using a combination of 1H NMR and a co-culture of endometrial cells the EMI.

Results: Twenty-six metabolites were identified and quantified in the EMI, nine metabolites reflected the sex of the embryo rather than their presence.

Conclusions: 1H NMR is sensitive enough to perform quantitative analysis of sex-induced differences in the EMI. These results may help to understand the embryo-maternal dialogue on the basis of embryonic sex.

Keywords: 1H NMR; Bovine; Embryo; Endometrial cells; Sex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Embryo, Mammalian / metabolism*
Female
Male
Maternal-Fetal Relations*
Metabolomics*
Morula / metabolism*
Proton Magnetic Resonance Spectroscopy