Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

Jianglai Wu; Yajie Liang; Shuo Chen; Ching-Lung Hsu; Mariya Chavarha; Stephen W Evans; Dongqing Shi; Michael Z Lin; Kevin K Tsia; Na Ji

doi:10.1038/s41592-020-0762-7

Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

Nat Methods. 2020 Mar;17(3):287-290. doi: 10.1038/s41592-020-0762-7. Epub 2020 Mar 2.

Authors

Jianglai Wu^{1

2

3}, Yajie Liang³, Shuo Chen¹, Ching-Lung Hsu³, Mariya Chavarha⁴, Stephen W Evans⁴, Dongqing Shi⁴, Michael Z Lin⁴, Kevin K Tsia⁵, Na Ji^{6

7

8

9

10}

Affiliations

¹ Department of Physics, University of California, Berkeley, CA, USA.
² Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, China.
³ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
⁴ Department of Bioengineering, Stanford University, Stanford, CA, USA.
⁵ Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, China. tsia@hku.hk.
⁶ Department of Physics, University of California, Berkeley, CA, USA. jina@berkeley.edu.
⁷ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. jina@berkeley.edu.
⁸ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. jina@berkeley.edu.
⁹ Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹⁰ Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. jina@berkeley.edu.

Abstract

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / diagnostic imaging*
Calcium / metabolism
Cells, Cultured
Computational Biology
Female
Glutamic Acid / metabolism
Lasers
Male
Membrane Potentials
Mice
Mice, Transgenic
Microscopy, Fluorescence, Multiphoton / methods*
Neurons / physiology*
Optics and Photonics
Photons*
Rats
Software

Substances

Glutamic Acid
Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding