Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1)

Soichiro Hayashi; Yoshiyuki Osada; Kazuki Miura; Siro Simizu

doi:10.1016/j.bbrep.2020.100734

Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1)

Biochem Biophys Rep. 2020 Jan 30:21:100734. doi: 10.1016/j.bbrep.2020.100734. eCollection 2020 Mar.

Authors

Soichiro Hayashi¹, Yoshiyuki Osada¹, Kazuki Miura¹, Siro Simizu¹

Affiliation

¹ Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan.

Abstract

Vasculogenic mimicry (VM) promotes tumor migration, metastasis, and invasion in various types of cancer, but the relationship between VM and these phenotypes remains undefined. In this study, we examined carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) as a novel target of VM. We found that ectopic expression of CEACAM1 in HT1080 human fibrosarcoma cells suppressed the formation of a VM-like network. Further, cell migration and proliferation were abated by the introduction of CEACAM1 into HT1080 cells. Conversely, knockout (KO) of the CEACAM1 gene in SK-MEL-28 melanoma cells, which normally express high levels of CEACAM1, inhibited formation of a VM-like network, which was covered on reintroduction of CEACAM1. These results suggest that CEACAM1 differentially regulates formation of the VM-like network between cancer cell types and implicate CEACAM1 as a novel therapeutic target in malignant cancer.

Keywords: CEACAM1; CEACAM1, carcinoembryonic antigen cell adhesion molecule 1; Cell migration; Cell proliferation; DMEM, Dulbecco's modified Eagle's medium; Fibrosarcoma; Melanoma; PBS, phosphate-buffered saline; SDS, sodium dodecyl sulfate; VM, vasculogenic mimicry; Vasculogenic mimicry.