Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing

Jinjing Li; Shunyan Hong; Wanjin Chen; Erwei Zuo; Hui Yang

doi:10.1016/j.jgg.2019.11.002

Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing

J Genet Genomics. 2019 Nov 20;46(11):513-521. doi: 10.1016/j.jgg.2019.11.002. Epub 2019 Nov 22.

Authors

Jinjing Li¹, Shunyan Hong¹, Wanjin Chen², Erwei Zuo³, Hui Yang⁴

Affiliations

¹ Department of Neurology and Institute of Neurology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China; Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, 350005, China.
² Department of Neurology and Institute of Neurology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China; Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, 350005, China. Electronic address: wanjinchen75@fjmu.edu.cn.
³ Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China. Electronic address: erweizuo@163.com.
⁴ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address: huiyang@ion.ac.cn.

PMID: 31911131
DOI: 10.1016/j.jgg.2019.11.002

Abstract

CRISPR-mediated genome editing is a revolutionary technology for genome manipulation that uses the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, discuss the merits and limitations of off-target detection methods, and provide potential implications for further gene editing research.

Keywords: Base editors; CRISPR-Cas9; Gene editing; Off-target.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

CRISPR-Associated Protein 9 / genetics
Chromatin Immunoprecipitation
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Gene Editing / methods*
High-Throughput Nucleotide Sequencing
Humans
RNA, Guide, CRISPR-Cas Systems / genetics
Whole Genome Sequencing / methods

Substances

RNA, Guide, CRISPR-Cas Systems
CRISPR-Associated Protein 9