Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein

Methods Mol Biol. 2020:2099:39-50. doi: 10.1007/978-1-0716-0211-9_4.

Abstract

Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD.

Keywords: Crystallization; MERS-CoV; RBD; Spike; Structure determination; X-ray crystallography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coronavirus Infections / virology*
  • Crystallization
  • Crystallography, X-Ray / methods*
  • Humans
  • Middle East Respiratory Syndrome Coronavirus / metabolism*
  • Protein Binding
  • Protein Domains
  • Receptors, Virus / chemistry*
  • Receptors, Virus / metabolism
  • Spike Glycoprotein, Coronavirus / chemistry*
  • Spike Glycoprotein, Coronavirus / isolation & purification
  • Spike Glycoprotein, Coronavirus / metabolism

Substances

  • Receptors, Virus
  • Spike Glycoprotein, Coronavirus