Brucein D (BD) is a naturally occurring major active quassinoid extracted from the Chinese medicinal herb Brucea javanica, which has been previously demonstrated to exhibit anticancer activities. The present study aimed to investigate the anticancer effects of BD on MDA-MB-231 cells, a human triple-negative breast cancer (TNBC) cell line. An MTT assay was performed to assess cell viability, whilst wound healing and Transwell assay were applied to measure cell migration and invasion, respectively. Western blot analysis was performed to assess the expression of E-cadherin, vimentin and β-catenin, which are proteins associated with epithelial-mesenchymal transformation (EMT), and PI3K, AKT and p-AKT, which are key components of the PI3K/AKT signaling pathway. BD was indicated to reduce cell viability in a dose- and time-dependent manner, whilst cell invasion and migration were also significantly inhibited in a dose-dependent manner. Western blot analysis demonstrated that BD treatment significantly upregulated the expression of E-cadherin and downregulated the expression of vimentin and β-catenin. Additionally, BD downregulated the expression of PI3K and reduced AKT phosphorylation. In conclusion, BD can inhibit MDA-MB-231 cell viability, migration and invasion, suggesting the potential use of BD for the treatment of TNBC.
Keywords: Bruceine D; PI3K/AKT; epithelial-mesenchymal transition; invasion; migration; triple-negative breast cancer.
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