Simple method of inducing sporulation by Apophysomyces elegans and Saksenaea vasiformis

J Clin Microbiol. 1988 Sep;26(9):1861-3. doi: 10.1128/jcm.26.9.1861-1863.1988.

Abstract

Apophysomyces elegans and Saksenaea vasiformis are notorious for their failure to sporulate on routine media. Agar blocks, permeated with the mycelia of A. elegans and S. vasiformis, were cut aseptically from 7-day-old colonies grown on Sabouraud dextrose agar and transferred to plates containing 20 ml of sterile distilled water supplemented with 0.2 ml of 10% filter-sterilized yeast extract solution. When the plates were incubated at 37 degrees C, all 5 isolates of A. elegans and all 10 isolates of S. vasiformis produced abundant, characteristic sporangia within 7 to 10 days. The method is simple to use and yields consistent results.

MeSH terms

  • Culture Media
  • Humans
  • Mucorales / physiology*
  • Spores, Fungal

Substances

  • Culture Media