Establishment of Mouse Models of Psoriasis with Blood Stasis Syndrome Complicated with Glucose and Lipid Metabolism Disorders

Ying Luo; Yi Ru; Huaibo Zhao; Liu Liu; Seokgyeong Hong; Xiaoying Sun; Le Kuai; Yi Lu; Meng Xing; Xi Chen; Jiankun Song; Yue Luo; Xiaoya Fei; Yaqiong Zhou; Hongjin Li; Bin Li; Xin Li

doi:10.1155/2019/6419509

Establishment of Mouse Models of Psoriasis with Blood Stasis Syndrome Complicated with Glucose and Lipid Metabolism Disorders

Evid Based Complement Alternat Med. 2019 Nov 12:2019:6419509. doi: 10.1155/2019/6419509. eCollection 2019.

Authors

Ying Luo¹, Yi Ru¹, Huaibo Zhao¹, Liu Liu¹, Seokgyeong Hong¹, Xiaoying Sun², Le Kuai¹, Yi Lu¹, Meng Xing¹, Xi Chen¹, Jiankun Song¹, Yue Luo¹, Xiaoya Fei¹, Yaqiong Zhou², Hongjin Li², Bin Li^{1

2}, Xin Li^{1

2}

Affiliations

¹ Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
² Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China.

Abstract

Background: Psoriasis has been reported as a high-risk factor for quality of life and survival rate in patients with metabolic disorder. However, there is no animal model for studying this disease. This study aimed to establish and evaluate mouse models of psoriasis with blood stasis syndrome (which is a key to psoriasis pathogenesis, according to Chinese Medicine) complicated with metabolic disorders.

Method: Forty-five C57BL/6 mice were randomly divided into the blank control (Control), psoriasis (Imiquimod (IMQ)), psoriasis with metabolic disorders (IMQ + streptozotocin (STZ)), psoriasis with blood stasis syndrome (BSS) (IMQ + BSS), and psoriasis with blood stasis syndrome complicated with metabolic disorders (IMQ + STZ + BSS) groups (n = 9 mice/group). Psoriasis lesions were induced using IMQ cream (on both the ears and back, except in the Control group). Mice of the IMQ + BSS group were fed a half-fat, high-sugar diet and stimulated with ice-water swimming every day. Mice of the IMQ + STZ group were fed a half-fat, high-sugar diet and injected with STZ. Mice of the IMQ + STZ + BSS group were subjected to the same treatments as the IMQ + STZ and IMQ + BSS groups. After induction, the mice in each group were observed for vital signs, ear thickness, body weight, and psoriasis area and severity index (PASI) score. The mice were fasted for 12 h before determination of related laboratory serum indexes. Dorsal skin lesions, aortic arch pathology sections, and signal transducer and activator of transcription 3 (STAT3) were examined by H&E staining and immunohistochemistry.

Results: Laboratory indexes in the four model groups were significantly different from those in the Control group (p < 0.01); indicators of the IMQ + STZ, IMQ + BSS, and IMQ + STZ + BSS groups showed varying degrees of difference from those of the IMQ group.

Conclusions: The established mouse models of psoriasis blood stasis syndrome complicated with glucose and lipid metabolism disorders met the clinical and Chinese Medicine characteristics, and thus they could be used as animal models in future studies of psoriasis complicated with glucose and lipid metabolism disorders.