Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalis

Kumaran Sundaram; Daniel P Miller; Anil Kumar; Yun Teng; Mohammed Sayed; Jingyao Mu; Chao Lei; Mukesh K Sriwastva; Lifeng Zhang; Jun Yan; Michael L Merchant; Liqing He; Yuan Fang; Shuangqin Zhang; Xiang Zhang; Juw W Park; Richard J Lamont; Huang-Ge Zhang

doi:10.1016/j.isci.2019.10.032

Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalis

iScience. 2019 Nov 22:21:308-327. doi: 10.1016/j.isci.2019.10.032. Epub 2019 Oct 21.

Authors

Kumaran Sundaram¹, Daniel P Miller², Anil Kumar¹, Yun Teng¹, Mohammed Sayed³, Jingyao Mu¹, Chao Lei¹, Mukesh K Sriwastva¹, Lifeng Zhang¹, Jun Yan¹, Michael L Merchant⁴, Liqing He⁵, Yuan Fang⁵, Shuangqin Zhang⁶, Xiang Zhang⁵, Juw W Park⁷, Richard J Lamont², Huang-Ge Zhang⁸

Affiliations

¹ James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA.
² Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY 40202, USA.
³ Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40202, USA.
⁴ Kidney Disease Program and Clinical Proteomics Center, University of Louisville, Louisville, KY, USA.
⁵ Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA.
⁶ Department of Medicine, University of Chicago, Chicago, IL 60637, USA.
⁷ Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40202, USA; KBRIN Bioinformatics Core, University of Louisville, Louisville, KY 40202, USA.
⁸ Robley Rex Veterans Affairs Medical Center, Louisville, KY 40206, USA; James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA. Electronic address: h0zhan17@louisville.edu.

Abstract

Plant exosomes protect plants against infection; however, whether edible plant exosomes can protect mammalian hosts against infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on the surface of P. gingivalis. Compared with PA (34:2), PA (34:1) did not interact with HBP35, indicating that the degree of unsaturation of PA plays a critical role in GELN-mediated interaction with HBP35. On binding to HBP35, pathogenic mechanisms of P. gingivalis were significantly reduced following interaction with GELN cargo molecules, including PA and miRs. These cargo molecules interacted with multiple pathogenic factors in the recipient bacteria simultaneously. Using edible plant exosome-like nanoparticles as a potential therapeutic agent to prevent/treat chronic periodontitis was further demonstrated in a mouse model.

Keywords: Bacteriology; Biochemistry; Biological Sciences; Immunology; Microbiology; Molecular Biology; Oral Microbiology.

Abstract

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