Genome-wide survey and expression analysis of the SLAC/SLAH gene family in pear (Pyrus bretschneideri) and other members of the Rosaceae

Guodong Chen; Xiaolong Li; Xin Qiao; Jiaming Li; Li Wang; Xiaobing Kou; Xiao Wu; Guoming Wang; Hao Yin; Peng Wang; Shaoling Zhang; Juyou Wu

doi:10.1016/j.ygeno.2018.07.004

Genome-wide survey and expression analysis of the SLAC/SLAH gene family in pear (Pyrus bretschneideri) and other members of the Rosaceae

Genomics. 2019 Sep;111(5):1097-1107. doi: 10.1016/j.ygeno.2018.07.004. Epub 2018 Jul 6.

Authors

Guodong Chen¹, Xiaolong Li¹, Xin Qiao¹, Jiaming Li¹, Li Wang¹, Xiaobing Kou¹, Xiao Wu¹, Guoming Wang¹, Hao Yin¹, Peng Wang¹, Shaoling Zhang¹, Juyou Wu²

Affiliations

¹ Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
² Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: juyouwu@njau.edu.cn.

PMID: 31533901
DOI: 10.1016/j.ygeno.2018.07.004

Abstract

S-type anion channels, which play important roles in plant anion (such as nitrate and chloride) transport, growth and development, abiotic stress responses and hormone signaling. However, there is far less information about this family in Rosaceae species. We performed a genome-wide analysis and identified SLAC/SLAH gene family members in pear (Pyrus bretschneideri) and four other species of Rosaceae. A total of 21 SLAC/SLAH genes were identified from the five Rosaceae species. Based on the structural characteristics and a phylogenetic analysis of these genes, the SLAC/SLAH gene family could be classified into three main groups. Transcriptome data demonstrated that PbrSLAC/SLAH genes were detected in all parts of the pear. PbrSLAC/SLAH genes were only located on the plasma membrane in transient expression experiments in Arabidopsis protoplasts cells. These results provide valuable information that increases our understanding of the evolution, expression and functions of the SLAC/SLAH gene family in higher plants.

Keywords: Pear (Pyrus bretschneideri); Rosaceae; Slow anion channel; Subcellular localization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anions / metabolism
Ion Channels / chemistry
Ion Channels / genetics*
Ion Channels / metabolism
Nitrates / metabolism
Phylogeny
Plant Proteins / chemistry
Plant Proteins / genetics*
Plant Proteins / metabolism
Pyrus / classification
Pyrus / genetics*
Transcriptome

Substances

Anions
Ion Channels
Nitrates
Plant Proteins