Novel Gain-of-Function Mutation in Stat1 Sumoylation Site Leads to CMC/CID Phenotype Responsive to Ruxolitinib

J Clin Immunol. 2019 Nov;39(8):776-785. doi: 10.1007/s10875-019-00687-4. Epub 2019 Sep 11.

Abstract

Mutations in the coiled-coil and DNA-binding domains of STAT1 lead to delayed STAT1 dephosphorylation and subsequently gain-of-function. The associated clinical phenotype is broad and can include chronic mucocutaneous candidiasis (CMC) and/or combined immunodeficiency (CID). We report a case of CMC/CID in a 10-year-old boy due to a novel mutation in the small ubiquitin molecule (SUMO) consensus site at the C-terminal region of STAT1 leading to gain-of-function by impaired sumoylation. Immunodysregulatory features of disease improved after Janus kinase inhibitor (jakinib) treatment. Functional testing after treatment confirmed reversal of the STAT1 hyper-phosphorylation and downstream transcriptional activity. IL-17 and IL-22 production was, however, not restored with jakinib therapy (ruxolitinib), and the patient remained susceptible to opportunistic infection. In conclusion, a mutation in the SUMO consensus site of STAT1 can lead to gain-of-function that is reversible with jakinib treatment. However, full immunocompetence was not restored, suggesting that this treatment strategy might serve well as a bridge to definitive therapy such as hematopoietic stem cell transplant rather than a long-term treatment option.

Keywords: STAT1; chronic mucocutaneous candidiasis; combined immunodeficiency; sumoylation.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candidiasis, Chronic Mucocutaneous / diagnosis
  • Candidiasis, Chronic Mucocutaneous / drug therapy
  • Candidiasis, Chronic Mucocutaneous / genetics*
  • Child
  • Gain of Function Mutation
  • Humans
  • Janus Kinases / antagonists & inhibitors
  • Male
  • Nitriles
  • Primary Immunodeficiency Diseases / diagnosis
  • Primary Immunodeficiency Diseases / drug therapy
  • Primary Immunodeficiency Diseases / genetics*
  • Pyrazoles / pharmacology
  • Pyrazoles / therapeutic use*
  • Pyrimidines
  • STAT1 Transcription Factor / genetics*
  • Sumoylation / genetics
  • Treatment Outcome

Substances

  • Nitriles
  • Pyrazoles
  • Pyrimidines
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • ruxolitinib
  • Janus Kinases