Mito-Specific Ratiometric Terbium(III)-Complex-Based Luminescent Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay

Yingzhe Wang; Boya Li; Xuerui Song; Rong Shen; Degui Wang; Yang Yang; Yan Feng; Chen Cao; Guolin Zhang; Weisheng Liu

doi:10.1021/acs.analchem.9b03024

Mito-Specific Ratiometric Terbium(III)-Complex-Based Luminescent Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay

Anal Chem. 2019 Oct 1;91(19):12422-12427. doi: 10.1021/acs.analchem.9b03024. Epub 2019 Sep 20.

Authors

Yingzhe Wang¹, Boya Li², Xuerui Song¹, Rong Shen², Degui Wang², Yang Yang¹, Yan Feng¹, Chen Cao¹, Guolin Zhang¹, Weisheng Liu¹

Affiliations

¹ Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering , Lanzhou University , Lanzhou 730000 , P. R. China.
² Department of Anatomy and Histology, School of Basic Medical Sciences , Lanzhou University , Lanzhou 730000 , P. R. China.

PMID: 31496225
DOI: 10.1021/acs.analchem.9b03024

Abstract

The level of peroxynitrite (ONOO^-) is closely related to the pathogenesis of a series of diseases. Hence, the accurate sensing of ONOO^- is very important for a better understanding of its biological functions. Here, a time-resolved ratiometric nanoprobe (TD-DC) composed of an energy donor unit (Tb(DPA)₃, where DPA signifies a dipicolinate dianion ligand) and an energy receptor unit (DC) was designed. The response of TD-DC toward ONOO^- in vitro was assessed by luminescence intensity and lifetime. The results demonstrated that TD-DC could precisely (39 nM) detect ONOO^- with high selectivity and efficiency (29 s). TD-DC was further successfully employed in sensing endogenous ONOO^- in A-375 cells. Moreover, it could specifically localize in the mitochondrion, where endogenous ONOO^- is mainly generated. The above results revealed that TD-DC is efficient and useful for real-time detection of ONOO^- at subcellular levels.

Publication types

Research Support, Non-U.S. Gov't