PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1

Xu Qian; Xinjian Li; Zhumei Shi; Yan Xia; Qingsong Cai; Daqian Xu; Lin Tan; Linyong Du; Yanhua Zheng; Dan Zhao; Chuanbao Zhang; Philip L Lorenzi; Yongping You; Bing-Hua Jiang; Tao Jiang; Haitao Li; Zhimin Lu

doi:10.1016/j.molcel.2019.08.006

PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1

Mol Cell. 2019 Nov 7;76(3):516-527.e7. doi: 10.1016/j.molcel.2019.08.006. Epub 2019 Sep 3.

Authors

Xu Qian¹, Xinjian Li², Zhumei Shi³, Yan Xia⁴, Qingsong Cai⁴, Daqian Xu⁴, Lin Tan⁵, Linyong Du⁶, Yanhua Zheng⁴, Dan Zhao⁷, Chuanbao Zhang⁸, Philip L Lorenzi⁵, Yongping You⁹, Bing-Hua Jiang¹⁰, Tao Jiang⁸, Haitao Li⁷, Zhimin Lu¹¹

Affiliations

¹ Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China; Institute for Brain Tumors, Jiangsu Key Lab of Cancer Biomarkers, Prevention, and Treatment, Jiangsu Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China; Brain Tumor Center and Department of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. Electronic address: xqianmedres@njmu.edu.cn.
² CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
³ Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
⁴ Brain Tumor Center and Department of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
⁵ Department of Bioinformatics & Computational Biology, Proteomics and Metabolomics Core Facility, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
⁶ School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
⁷ MOE Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
⁸ Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
⁹ Institute for Brain Tumors, Jiangsu Key Lab of Cancer Biomarkers, Prevention, and Treatment, Jiangsu Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China; Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
¹⁰ The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China; Department of Pathology, University of Iowa, Iowa City, IA 52242, USA.
¹¹ Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310029, China. Electronic address: zhiminlu@zju.edu.cn.

PMID: 31492635
DOI: 10.1016/j.molcel.2019.08.006

Abstract

The PTEN tumor suppressor is frequently mutated or deleted in cancer and regulates glucose metabolism through the PI3K-AKT pathway. However, whether PTEN directly regulates glycolysis in tumor cells is unclear. We demonstrate here that PTEN directly interacts with phosphoglycerate kinase 1 (PGK1). PGK1 functions not only as a glycolytic enzyme but also as a protein kinase intermolecularly autophosphorylating itself at Y324 for activation. The protein phosphatase activity of PTEN dephosphorylates and inhibits autophosphorylated PGK1, thereby inhibiting glycolysis, ATP production, and brain tumor cell proliferation. In addition, knockin expression of a PGK1 Y324F mutant inhibits brain tumor formation. Analyses of human glioblastoma specimens reveals that PGK1 Y324 phosphorylation levels inversely correlate with PTEN expression status and are positively associated with poor prognosis in glioblastoma patients. This work highlights the instrumental role of PGK1 autophosphorylation in its activation and PTEN protein phosphatase activity in governing glycolysis and tumorigenesis.

Keywords: PGK1; PTEN; autophosphorylation; glycolysis; tumorigenesis.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / metabolism
Animals
Brain Neoplasms / enzymology*
Brain Neoplasms / genetics
Brain Neoplasms / pathology
Cell Line, Tumor
Cell Proliferation
Female
Glioblastoma / enzymology*
Glioblastoma / genetics
Glioblastoma / pathology
Glucose / metabolism*
Glycolysis*
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
PTEN Phosphohydrolase / genetics
PTEN Phosphohydrolase / metabolism*
Phosphoglycerate Kinase / genetics
Phosphoglycerate Kinase / metabolism*
Phosphorylation
Prognosis
Signal Transduction
Time Factors
Tumor Burden
Tyrosine

Substances

Tyrosine
Adenosine Triphosphate
PGK1 protein, human
Pgk1 protein, mouse
Phosphoglycerate Kinase
PTEN Phosphohydrolase
PTEN protein, human
Pten protein, mouse
Glucose