Inhibition of anaerobic biological sulfate reduction process by copper precipitates

Abstract

The single-stage biological sulfate reduction process for treatment of heavy metal laden wastewater is a promising treatment method, but the formation of metal precipitates has been suggested to be inhibitory to the activity of sulfate reducers. The present study examined the impact of copper (Cu) precipitates on anaerobic biological sulfate reduction in semi continuous stirred tank reactors (SCSTRs) at 35 ± 2 °C. The results show that Cu precipitates significantly affected the sulfate reduction process. At an HRT of 50 days, steady-state sulfate reduction was approximately 55% at influent Cu concentrations of 0 (control) and 200 mg/L, which reduced to approximately 39% at influent Cu concentration of 400 mg/L. Microbial population (measured as volatile suspended solids) and rate of sulfate reduction were also affected, with reductions observed even at influent Cu of 200 mg/L. Copper precipitates also affected the microbial community diversity distribution.

Keywords: Biological sulfate reduction; Copper precipitates; Inhibitory effect; Metal laden wastewater; Semi continuous stirred tank reactors; Sulfate reducing bacteria.