Variable loss of CD30 expression by immunohistochemistry in recurrent cutaneous CD30+ lymphoid neoplasms treated with brentuximab vedotin

J Cutan Pathol. 2019 Nov;46(11):823-829. doi: 10.1111/cup.13545. Epub 2019 Aug 2.

Abstract

Aims: Brentuximab vedotin is a monoclonal anti-CD30 antibody-drug conjugate that has been used to treat a variety of CD30+ neoplasms. The phenomenon of antigen loss has been observed in patients treated with the anti-CD20 antibody rituximab. This study seeks to assess for antigen loss in the setting of recurrent CD30+ neoplasms treated with brentuximab vedotin.

Methods: We report nine cases of persistent/recurrent cutaneous CD30+ lymphoid neoplasms that demonstrated variable CD30 expression after treatment with brentuximab vedotin. Cases include MF (n = 6), cutaneous T-cell lymphoma, not otherwise specified (n = 1), and anaplastic large cell lymphoma (ALCL), both primary (n = 1) and systemic (n = 1).

Results: Immunohistochemical staining revealed decreased CD30 expression following brentuximab vedotin therapy in seven of nine cases. In these seven cases, the pre-treatment percent of tumor cells staining for CD30 ranged from 10% to 100% (mean 50.0%, SD 27.8%), compared to 5% to 50% (mean 14.5%, SD 14.8%, P = 0.003) at recurrence.

Conclusions: This case series highlights the finding that CD30 positivity can be variable in recurrences after treatment with anti-CD30 antibodies. This serves to raise awareness of the phenomenon of antigen loss after treatment with brentuximab vedotin and underscores the utility of performing multiple biopsies and/or employing molecular diagnostic techniques in patients with recurrent/persistent disease.

Keywords: CD30+ cutaneous T-cell lymphoma; anaplastic large cell lymphoma; brentuximab; loss of CD30; mycosis fungoides.

Publication types

  • Case Reports
  • Clinical Trial

MeSH terms

  • Aged
  • Brentuximab Vedotin / administration & dosage*
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Immunohistochemistry
  • Ki-1 Antigen / biosynthesis*
  • Lymphoma, Large-Cell, Anaplastic* / drug therapy
  • Lymphoma, Large-Cell, Anaplastic* / metabolism
  • Lymphoma, Large-Cell, Anaplastic* / pathology
  • Lymphoma, T-Cell, Cutaneous* / drug therapy
  • Lymphoma, T-Cell, Cutaneous* / metabolism
  • Lymphoma, T-Cell, Cutaneous* / pathology
  • Male
  • Middle Aged
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Recurrence, Local / drug therapy
  • Neoplasm Recurrence, Local / metabolism
  • Neoplasm Recurrence, Local / pathology
  • Skin Neoplasms* / drug therapy
  • Skin Neoplasms* / metabolism
  • Skin Neoplasms* / pathology

Substances

  • Ki-1 Antigen
  • Neoplasm Proteins
  • Brentuximab Vedotin