UPLC/MS/MS assay for the simultaneous determination of seven antibiotics in human serum-Application to pediatric studies

J Pharm Biomed Anal. 2019 Sep 10:174:256-262. doi: 10.1016/j.jpba.2019.03.004. Epub 2019 Mar 7.

Abstract

A rapid and highly sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) assay was developed for quantification of 7 antibiotics in low sample volumes (50 μL): amoxicillin, azithromycin, cefotaxime, ciprofloxacin, meropenem, metronidazole and piperacillin, for both routine monitoring and pharmacokinetic studies. After protein precipitation by acetonitrile, the antibiotics were separated on an Acquity UPLC HSS T3 column (run time, 4 min). The mobile phase consisted of a mixture of (A) ammonium acetate (pH 2.4; 5 mM) and (B) acetonitrile acidified with 0.1% formic acid, delivered at 500 μl/min in a gradient elution mode. Total time run was 2.75 min. Ions were detected in the turbo-ion-spray-positive and multiple-reaction-monitoring modes. The assay was accurate and reproductible for the quantification of the seven antibiotics in serum samples over large concentration ranges.

Keywords: Antibiotics; Pediatrics; Pharmacokinetics; UPLC-MS/MS.

MeSH terms

  • Adolescent
  • Amoxicillin / blood
  • Anti-Bacterial Agents / blood*
  • Azithromycin / blood
  • Blood Chemical Analysis / methods*
  • Calibration
  • Cefotaxime / blood
  • Child
  • Child, Preschool
  • Chromatography, High Pressure Liquid / methods*
  • Ciprofloxacin / blood
  • Escherichia coli Infections / drug therapy
  • Humans
  • Infant
  • Infant, Newborn
  • Leukemia, Myeloid, Acute / complications
  • Leukemia, Myeloid, Acute / microbiology
  • Limit of Detection
  • Male
  • Meropenem / blood
  • Metronidazole / blood
  • Pediatrics
  • Piperacillin / blood
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*

Substances

  • Anti-Bacterial Agents
  • Metronidazole
  • Ciprofloxacin
  • Amoxicillin
  • Azithromycin
  • Meropenem
  • Cefotaxime
  • Piperacillin