In Vivo Measurement of H2S, Polysulfides, and "SSNO- Mix"-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform

Frantisek Kristek; Marian Grman; Karol Ondrias

doi:10.1007/978-1-4939-9528-8_8

In Vivo Measurement of H₂S, Polysulfides, and "SSNO^- Mix"-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform

Methods Mol Biol. 2019:2007:109-124. doi: 10.1007/978-1-4939-9528-8_8.

Authors

Frantisek Kristek¹, Marian Grman², Karol Ondrias²

Affiliations

¹ Institute of Normal and Pathological Physiology, Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia. Frantisek.Kristek@savba.sk.
² Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.

PMID: 31148109
DOI: 10.1007/978-1-4939-9528-8_8

Abstract

The chapter describes protocols and pitfalls in in vivo studies of drug effects on anesthetized rats. It focuses on the preparation of Na₂S, Na₂S₄, and "SSNO^- mix" solutions for rat intravenous administration, surgical preparation of jugular vein for drug administration, and preparation of carotid and tail arteries for recording of arterial pulse waveform (APW) at high resolution. It describes evaluation of ten hemodynamic parameters from APW and measurement of apparent pulse wave velocity.

Keywords: Arterial pulse waveform; Carotid artery; H2S; Hemodynamic parameters; Jugular vein; Na2S4; Rat blood pressure; SSNO.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carotid Arteries / physiopathology*
Hemodynamics / drug effects*
Hydrogen Sulfide* / pharmacokinetics
Hydrogen Sulfide* / pharmacology
Male
Pulse Wave Analysis*
Rats
Rats, Wistar
Sulfides* / pharmacokinetics
Sulfides* / pharmacology

Substances

Sulfides
polysulfide
Hydrogen Sulfide