A PET study in healthy subjects of brain exposure of 11C-labelled osimertinib - A drug intended for treatment of brain metastases in non-small cell lung cancer

J Cereb Blood Flow Metab. 2020 Apr;40(4):799-807. doi: 10.1177/0271678X19843776. Epub 2019 Apr 20.

Abstract

Osimertinib is a tyrosine kinase inhibitor (TKI) of the mutated epidermal growth factor receptor (EGFRm) with observed efficacy in patients with brain metastases. Brain exposure and drug distribution in tumor regions are important criteria for evaluation and confirmation of CNS efficacy. The aim of this PET study was therefore to determine brain distribution and exposure of 11C-labelled osimertinib administered intravenously in subjects with an intact blood-brain barrier. Eight male healthy subjects (age 52 ± 8 years) underwent one PET measurement with 11C-osimertinib. The pharmacokinetic parameters Cmax(brain) (standardized uptake value), Tmax(brain) and AUC0-90 minbrain/blood ratio were calculated. The outcome measure for 11C-osimertinib brain exposure was the total distribution volume (VT). 11C-osimertinib distributed rapidly to the brain, with higher uptake in grey than in white matter. Mean Cmax, Tmax and AUC0-90 minbrain/blood ratio were 1.5 (range 1-1.8), 13 min (range 5-30 min), and 3.8 (range 3.3-4.1). Whole brain and white matter VT were 14 mL×cm-3 (range 11-18) and 7 mL×cm-3 (range 5-12). This study in healthy volunteers shows that 11C-osimertinib penetrates the intact blood-brain barrier. The approach used further illustrates the role of molecular imaging in facilitating the development of novel drugs for the treatment of malignancies affecting the brain.

Keywords: Brain metastasis; PET; blood–brain barrier; epidermal growth factor receptor; microdose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylamides / administration & dosage
  • Acrylamides / pharmacokinetics*
  • Adult
  • Aniline Compounds / administration & dosage
  • Aniline Compounds / pharmacokinetics*
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / pharmacokinetics*
  • Blood-Brain Barrier / metabolism
  • Brain / diagnostic imaging*
  • Brain / metabolism
  • Brain Neoplasms / drug therapy*
  • Brain Neoplasms / secondary
  • Carbon Radioisotopes
  • Carcinoma, Non-Small-Cell Lung / drug therapy*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Healthy Volunteers
  • Humans
  • Injections, Intravenous
  • Magnetic Resonance Imaging / methods
  • Male
  • Middle Aged
  • Positron-Emission Tomography / methods*
  • Tissue Distribution

Substances

  • Acrylamides
  • Aniline Compounds
  • Antineoplastic Agents
  • Carbon Radioisotopes
  • Carbon-11
  • osimertinib