NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador

J E Villacís; M Bovera; D Romero-Alvarez; F Cornejo; V Albán; G Trueba; H F Dorn; J A Reyes

doi:10.1016/j.nmni.2019.100526

NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador

New Microbes New Infect. 2019 Mar 8:29:100526. doi: 10.1016/j.nmni.2019.100526. eCollection 2019 May.

Authors

J E Villacís^{1

2}, M Bovera³, D Romero-Alvarez⁴, F Cornejo³, V Albán⁵, G Trueba⁵, H F Dorn³, J A Reyes^{6

5}

Affiliations

¹ Carrera de Bioquímica Clínica, Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador.
² Centro de Referencia Nacional de Resistencia a los Antimicrobianos Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Quito, Ecuador.
³ Hospital de los Valles, Quito, Ecuador.
⁴ Department of Ecology & Evolutionary Biology and Biodiversity Institute, University of Kansas, Lawrence, KS, USA.
⁵ Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco, Quito, Pichincha, Ecuador.
⁶ Facultad de Ciencias Químicas, Universidad Central del Ecuador, Quito, Pichincha, Ecuador.

Abstract

Objectives: To describe a clinical case of Acinetobacter baumannii sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador.

Methods: We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an A. baumannii isolate. We used synergy with the imipenem-EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing.

Results: Molecular characterization revealed the presence of A. baumannii ST32 harbouring the bla _NDM-1 gene in Ecuador. The bla _NDM-1 gene was isolated through PCR and amplified from a purified plasmid.

Conclusions: To the best of our knowledge, this is the first report of A. baumannii ST32 harbouring the bla _NDM-1 gene.

Keywords: Acinetobacter baumannii; MSLT; antibiotic resistance; blaNDM-1; sequence type 32.