Programming a split G-quadruplex in a DNA nanocage and its microRNA imaging in live cells

Chem Commun (Camb). 2019 Apr 25;55(35):5131-5134. doi: 10.1039/c9cc02096a.

Abstract

A novel approach to program target-responsive devices by incorporating the split G4 motifs in a DNA nanocage has been developed. The rigid prism outcompetes the flexible one in reaction kinetics and signal/background ratios, which can be easily internalized by cells and successfully applied in microRNA imaging in live cells.

MeSH terms

  • Benzothiazoles / chemistry
  • Cell Line, Tumor
  • DNA / chemistry*
  • DNA / genetics
  • Fluorescence Resonance Energy Transfer / methods
  • Fluorescent Dyes / chemistry
  • G-Quadruplexes*
  • Humans
  • Intercalating Agents / chemistry
  • Kinetics
  • MicroRNAs / analysis*
  • MicroRNAs / genetics
  • Microscopy, Confocal / methods
  • Nanoparticles / chemistry*
  • Nucleic Acid Hybridization

Substances

  • Benzothiazoles
  • Fluorescent Dyes
  • Intercalating Agents
  • MIRN122 microRNA, human
  • MicroRNAs
  • thioflavin T
  • DNA