The Application of Multi-Walled Carbon Nanotubes in Bone Tissue Repair Hybrid Scaffolds and the Effect on Cell Growth In Vitro

Jie Xu; Xueyan Hu; Siyu Jiang; Yiwei Wang; Roxanne Parungao; Shuangshuang Zheng; Yi Nie; Tianqing Liu; Kedong Song

doi:10.3390/polym11020230

The Application of Multi-Walled Carbon Nanotubes in Bone Tissue Repair Hybrid Scaffolds and the Effect on Cell Growth In Vitro

Polymers (Basel). 2019 Feb 1;11(2):230. doi: 10.3390/polym11020230.

Authors

Jie Xu¹, Xueyan Hu², Siyu Jiang³, Yiwei Wang⁴, Roxanne Parungao⁵, Shuangshuang Zheng⁶, Yi Nie^{7

8}, Tianqing Liu⁹, Kedong Song¹⁰

Affiliations

¹ State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. xujie172@mail.dlut.edu.cn.
² State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Huxueyan@mail.dlut.edu.cn.
³ State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. syjiang0226@mail.dlut.edu.cn.
⁴ Burns Research Group, ANZAC Research Institute, Concord, University of Sydney, Sydney, NSW 2139, Australia. yiweiwang@anzac.edu.au.
⁵ Burns Research Group, ANZAC Research Institute, Concord, University of Sydney, Sydney, NSW 2139, Australia. rpar4161@uni.sydney.edu.au.
⁶ Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, China. sszheng@ipezz.ac.cn.
⁷ Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, China. ynie@ipe.ac.cn.
⁸ Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. ynie@ipe.ac.cn.
⁹ State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. liutq@dlut.edu.cn.
¹⁰ State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. kedongsong@dlut.edu.cn.

Abstract

In this study, composite scaffolds with different multi-walled carbon nanotubes (MWCNTs) content were prepared by freeze-drying. These scaffolds were characterized by scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), porosity, hydrophilicity, mechanical strength, and degradation. The MWCNTs scaffolds were structurally sound and had porous structures that offered ample space for adherence, proliferation, and differentiation of MC3T3-E1 cells, and also supported the transport of nutrients and metabolic waste. CS/Gel/nHAp/0.3%MWCNTs scaffolds provided the best outcomes in terms of scaffold porosity, hydrophilicity, and degradation rate. However, CS/Gel/nHAp/0.6%MWCNTs scaffolds were found to support the optimal growth, homogenous distribution, and biological activity of MC3T3-E1 cells. The excellent properties of CS/Gel/nHAp/0.6%MWCNTs scaffolds for the adhesion, proliferation, and osteogenesis differentiation of MC3T3-E1 cells in vitro highlights the potential applications of this scaffold in bone tissue regeneration.

Keywords: CS/Gel/nHAp; MC3T3-E1 cells; MWCNTs; bone tissue engineering.

Abstract

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