Transplantation of umbilical cord-derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice

In Vitro Cell Dev Biol Anim. 2019 Apr;55(4):302-311. doi: 10.1007/s11626-019-00337-4. Epub 2019 Feb 28.

Abstract

Premature ovarian failure (POF) is a refractory disease; one of the most important goals of treatment is to improve fertility. In the study, collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells (collagen/UC-MSCs) transplantation in POF mice preserved ovarian function, as supported by increased estrogen (E2) and anti-Mullerian hormone (AMH) levels, increased ovarian volume, and an increased number of antral follicles. Immunohistochemistry results of Ki67 indicated transplantation of collagen/UC-MSCs promoted granulosa cell proliferation, which is crucial to oocyte maturation and follicular development. Additionally, transplantation of collagen/UC-MSCs significantly promoted ovarian angiogenesis with the increased expression of CD31. In general, collagen/UC-MSCs transplantation probably is an effective therapeutic strategy of POF.

Keywords: Collagen scaffold; Ovarian function; POF; UC-MSCs.

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Collagen / pharmacology*
  • Disease Models, Animal
  • Estrous Cycle / drug effects
  • Female
  • Granulosa Cells / drug effects
  • Granulosa Cells / metabolism
  • Hormones / metabolism
  • Humans
  • Infant, Newborn
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / metabolism
  • Mice, Inbred C57BL
  • Neovascularization, Physiologic / drug effects
  • Ovary / blood supply
  • Ovary / drug effects
  • Ovary / pathology
  • Ovary / physiopathology*
  • Primary Ovarian Insufficiency / physiopathology*
  • Primary Ovarian Insufficiency / therapy*
  • Tissue Scaffolds / chemistry*
  • Umbilical Cord / cytology*

Substances

  • Hormones
  • Collagen