Nucleic Acid Isolation and Quality Control

Ling Dong; Janice Yoshizawa; Xinmin Li

doi:10.1007/978-1-4939-8935-5_28

Nucleic Acid Isolation and Quality Control

Methods Mol Biol. 2019:1897:325-343. doi: 10.1007/978-1-4939-8935-5_28.

Authors

Ling Dong¹, Janice Yoshizawa¹, Xinmin Li²

Affiliations

¹ Technology Center for Genomics and Bioinformatics (TCGB), Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
² Technology Center for Genomics and Bioinformatics (TCGB), Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA, USA. xinminli@mednet.ucla.edu.

PMID: 30539456
DOI: 10.1007/978-1-4939-8935-5_28

Abstract

This chapter describes the most practical manual and automated methods of isolating genomic DNA and RNA from different sources. It also summarizes currently popular methods of quality control (QC) for genomic DNA, RNA, and next generation sequencing (NGS) libraries.

Keywords: Genomic DNA isolation; Genomic DNA quality control; Next generation sequencing library quality control; RNA isolation; RNA quality control.

Publication types

Review

MeSH terms

Biological Specimen Banks / trends*
Computational Biology
DNA / genetics
DNA / isolation & purification*
Genomics
High-Throughput Nucleotide Sequencing / methods*
Humans
Quality Control
RNA / genetics
RNA / isolation & purification*
Sequence Analysis, DNA

Substances

RNA
DNA