Proteomic Profiling for Target Identification of Biologically Active Small Molecules Using 2D DIGE

Methods Mol Biol. 2019:1888:127-139. doi: 10.1007/978-1-4939-8891-4_7.

Abstract

Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. This system compares the similarities in protein expression of 296 spots detected in the gel among the test compounds.

Keywords: Multivariate analysis; Profiling; Proteomics; Target identification of compounds; Two-dimensional florescent electrophoresis (2D DIGE).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Data Analysis
  • Drug Discovery / methods*
  • Fluorescent Dyes
  • Humans
  • Proteome*
  • Proteomics / methods*
  • Small Molecule Libraries
  • Staining and Labeling
  • Two-Dimensional Difference Gel Electrophoresis*

Substances

  • Fluorescent Dyes
  • Proteome
  • Small Molecule Libraries