Serological evidence of human infection by bat orthoreovirus in Singapore

J Med Virol. 2019 Apr;91(4):707-710. doi: 10.1002/jmv.25355. Epub 2018 Nov 28.

Abstract

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays. Our findings confirm that PRV spillover into human populations is relatively common in this region of the world.

Keywords: bat; luciferase immunoprecipitation system (LIPS); orthoreovirus; spillover; virus neutralization test (VNT).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Antibodies, Viral / blood*
  • Female
  • Humans
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Orthoreovirus / immunology*
  • Reoviridae Infections / epidemiology*
  • Reoviridae Infections / virology
  • Seroepidemiologic Studies
  • Serologic Tests / methods*
  • Singapore / epidemiology
  • Young Adult
  • Zoonoses / epidemiology*
  • Zoonoses / virology

Substances

  • Antibodies, Viral