Rearrangement of immunoglobulin heavy chain genes in human T leukaemic cells shows preferential utilization of the D segment (DQ52) nearest to the J region

EMBO J. 1986 Dec 20;5(13):3467-73. doi: 10.1002/j.1460-2075.1986.tb04671.x.

Abstract

The DNA rearrangements leading to the assembly of genes coding for the immunoglobulin heavy chain (IgH) in B cells and the T cell receptor for antigen in T cells are not completely lineage specific. This probably reflects the use of a common recombinase by IgH and the T cell receptor. This paper describes novel observations on the nature of these cross-lineage rearrangements. A high proportion (though not all) IgH rearrangements in human T leukaemic cells involve the D segment nearest to the J region (DQ52). This same D segment is not involved in B cell IgH rearrangements with one important exception, namely a proportion of B cell leukaemic clones with the most primitive B cell precursor phenotype. These observations have potentially important implications for early lymphoid cell differentiation and in particular support the idea that the 3' D plus J region might lie within a limited window of accessibility of the IgH gene in precursor lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Restriction Enzymes
  • Genes*
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin J-Chains / genetics*
  • Leukemia / genetics
  • Leukemia / immunology*
  • Nucleic Acid Hybridization
  • T-Lymphocytes / immunology*

Substances

  • Immunoglobulin Heavy Chains
  • Immunoglobulin J-Chains
  • DNA Restriction Enzymes