Generation of an induced pluripotent stem cell (iPSC) line from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Leu328Val) mutation

Stem Cell Res. 2018 Dec:33:6-9. doi: 10.1016/j.scr.2018.08.019. Epub 2018 Aug 31.

Abstract

Mutations in the KCNA2 gene, coding for the voltage-gated K+ channel Kv1.2, can cause developmental and epileptic encephalopathies. Kv1.2 channels play an important role in the repolarization phase of an action potential in nerve cells. Here, we reprogrammed human skin fibroblasts from a 13-year-old male patient with developmental and epileptic encephalopathy carrying a point mutation (c.982T>G, p.Leu328Val) in KCNA2 to human induced pluripotent stem cells (iPSCs) (HIHDNEi001-A). The cells maintained a normal karyotype and their pluripotency state was verified by the expression and staining of several pluripotency markers and capability to differentiate into all three germ layers.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Brain Diseases / genetics*
  • Humans
  • Induced Pluripotent Stem Cells / metabolism*
  • Kv1.2 Potassium Channel / adverse effects*
  • Male
  • Mutation
  • Spasms, Infantile / genetics*

Substances

  • KCNA2 protein, human
  • Kv1.2 Potassium Channel

Supplementary concepts

  • Infantile Epileptic-Dyskinetic Encephalopathy