Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen

Weston B Struwe; Elena Chertova; Joel D Allen; Gemma E Seabright; Yasunori Watanabe; David J Harvey; Max Medina-Ramirez; James D Roser; Rodman Smith; David Westcott; Brandon F Keele; Julian W Bess Jr; Rogier W Sanders; Jeffrey D Lifson; John P Moore; Max Crispin

doi:10.1016/j.celrep.2018.07.080

Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen

Cell Rep. 2018 Aug 21;24(8):1958-1966.e5. doi: 10.1016/j.celrep.2018.07.080.

Authors

Weston B Struwe¹, Elena Chertova², Joel D Allen³, Gemma E Seabright⁴, Yasunori Watanabe⁴, David J Harvey⁵, Max Medina-Ramirez⁶, James D Roser², Rodman Smith², David Westcott², Brandon F Keele², Julian W Bess Jr², Rogier W Sanders⁷, Jeffrey D Lifson², John P Moore⁸, Max Crispin⁹

Affiliations

¹ Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, OX1 3TA, UK.
² AIDS and Cancer Virus Program, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
³ Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, UK.
⁴ Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK; Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, UK.
⁵ Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, UK; Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7FZ, UK.
⁶ Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
⁷ Department of Microbiology and Immunology, Weill Cornell Medical College, Cornell University, New York, NY, USA; Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
⁸ Department of Microbiology and Immunology, Weill Cornell Medical College, Cornell University, New York, NY, USA. Electronic address: jpm2003@med.cornell.edu.
⁹ Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK; Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, UK. Electronic address: max.crispin@soton.ac.uk.

Abstract

Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes. The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design. While SOSIP trimers are close structural and antigenic mimics of virion Env, the extent to which their glycan structures resemble ones on infectious viruses is undefined. Here, we compare the overall glycosylation of gp120 and gp41 subunits from BG505 (clade A) virions produced in a lymphoid cell line with those from recombinant BG505 SOSIP trimers, including CHO-derived clinical grade material. We also performed detailed site-specific analyses of gp120. Glycans relevant to key bnAb epitopes are generally similar on the recombinant SOSIP and virion-derived Env proteins, although the latter do contain hotspots of elevated glycan processing. Knowledge of native versus recombinant Env glycosylation will guide vaccine design and manufacturing programs.

Keywords: HIV; envelope glycoprotein; glycosylation; mass spectrometry.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Glycosylation
HIV-1 / immunology*
Humans
Virion / metabolism*

Abstract

Publication types

MeSH terms

Grants and funding