Cell Polarity and Division Symmetry Analyses in Transformed Blood Cells

Methods Mol Biol. 2018:1821:257-266. doi: 10.1007/978-1-4939-8612-5_18.

Abstract

Cdc42 was originally discovered as a key regulator of bud site assembly and polarity in S. cerevisiae. Recent genetic studies have shown that the function of Cdc42 in regulating cell polarity appears highly conserved from budding yeast to humans. The role of Cdc42 in hematopoietic cell transformation and leukemia progression has been studied in an acute myeloid leukemia model using the MLL-AF9 oncogene-induced transformation and a Cdc42 conditional gene-targeted mouse model. Here we describe the leukemia cell polarity and division symmetry assays in the context of leukemia cell fate determination.

Keywords: Cell fate; Cell polarity; Differentiation; Division symmetry; Self-renewal.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division*
  • Cell Polarity*
  • Cell Transformation, Neoplastic / genetics
  • Cell Transformation, Neoplastic / metabolism*
  • Cell Transformation, Neoplastic / pathology
  • Hematopoietic Stem Cells / metabolism*
  • Hematopoietic Stem Cells / pathology
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Humans
  • Leukemia, Myeloid, Acute / genetics
  • Leukemia, Myeloid, Acute / metabolism*
  • Leukemia, Myeloid, Acute / pathology
  • Mice
  • Neoplasms, Experimental / genetics
  • Neoplasms, Experimental / metabolism*
  • Neoplasms, Experimental / pathology
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Oncogene Proteins, Fusion / genetics
  • Oncogene Proteins, Fusion / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • cdc42 GTP-Binding Protein / genetics
  • cdc42 GTP-Binding Protein / metabolism
  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / genetics
  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / metabolism

Substances

  • Cdc42 protein, mouse
  • Homeodomain Proteins
  • Mixl1 protein, mouse
  • Mllt3 protein, mouse
  • Nuclear Proteins
  • Oncogene Proteins, Fusion
  • cdc42 GTP-Binding Protein
  • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae