Prevalence of virulence determinants and antibiotic resistance patterns of Enterococcus faecalis strains in patients with community-acquired urinary tract infections in Iran

Int J Environ Health Res. 2018 Dec;28(6):599-608. doi: 10.1080/09603123.2018.1497777. Epub 2018 Jul 25.

Abstract

The aim of this study was to characterize virulence factors and antibiotic resistance patterns in E. faecalis strains obtained from community-acquired urinary tract infections. A total of 70 E. faecalis isolates from Labbafinejad Hospital in Tehran were collected. Antibiotic resistance and virulence determinants were examined by phenotypic and molecular methods. Among 70 E. faecalis isolates, efba (97.1%), ace (95.7%), and gelE (94.3%) were the most prevalent virulence genes. The most common antibiotic resistance pattern was tetracycline (88.6%) and minocycline (87.1%). Multi-drug resistant phenotype was detected among 10% of them. Our results showed capability of E. faecalis strains for infection of the urinary tract in community. Involvement of virulence determinants in the pathogenesis of community acquired E. faecalis strains was proposed due to their high prevalence rates. Food producing animals were proposed as their environmental reservoirs, due to dominance of tetracycline resistance phenotype among them.

Keywords: Antibiotic resistance; Enterococcus faecalis; urinary tract infection; virulence factors.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents* / pharmacology
  • Bacterial Proteins / genetics
  • Child
  • Child, Preschool
  • Community-Acquired Infections / epidemiology
  • Community-Acquired Infections / microbiology*
  • Drug Resistance, Bacterial / drug effects
  • Drug Resistance, Bacterial / genetics*
  • Enterococcus faecalis / drug effects
  • Enterococcus faecalis / isolation & purification
  • Enterococcus faecalis / pathogenicity*
  • Enterococcus faecalis / physiology*
  • Female
  • Humans
  • Iran / epidemiology
  • Male
  • Microbial Sensitivity Tests
  • Microbial Viability / drug effects
  • Middle Aged
  • Prevalence
  • Urinary Tract Infections / epidemiology
  • Urinary Tract Infections / microbiology*
  • Virulence Factors / genetics*
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Virulence Factors