Coidentification of mcr-4.3 and blaNDM-1 in a Clinical Enterobacter cloacae Isolate from China

Antimicrob Agents Chemother. 2018 Sep 24;62(10):e00649-18. doi: 10.1128/AAC.00649-18. Print 2018 Oct.

Abstract

We describe the first report of a clinical colistin-resistant ST84 Enterobacter cloacae isolate coharboring mcr-4.3 (previously named mcr-4.2) and blaNDM-1 from a patient in China. The blaNDM-1-harboring IncX3 plasmid and the novel mcr-4.3-harboring ColE plasmid were completely sequenced. Although this isolate showed a high level of resistance to colistin, mcr-4.3 plasmid transformation, gene subcloning, susceptibility testing, and lipid A matrix-assisted laser desorption ionization mass spectrometry analysis indicated that mcr-4.3 itself does not confer resistance to colistin.

Keywords: NDM-1; carbapenem resistant; colistin; mcr-4; plasmid.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbapenems / pharmacology
  • China
  • Colistin / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics
  • Enterobacter cloacae / drug effects
  • Enterobacter cloacae / enzymology*
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Carbapenems
  • beta-Lactamases
  • beta-lactamase NDM-1
  • Colistin