Discovery of Arabidopsis UGT73C1 as a steviol-catalyzing UDP-glycosyltransferase with chemical probes

Chem Commun (Camb). 2018 Jun 26;54(52):7179-7182. doi: 10.1039/c7cc09951g.

Abstract

Chemoselective and affinity-based probes of steviol were applied to the discovery of glycosyltransferase activity and corresponding UDP-glycosyltransferase in Arabidopsis thaliana, which illustrates a simple strategy for rapidly harnessing minable novel biological parts from plants for synthetic biology.

MeSH terms

  • Arabidopsis / enzymology*
  • Biocatalysis
  • Diterpenes, Kaurane / chemistry
  • Diterpenes, Kaurane / metabolism*
  • Glycosyltransferases / chemistry
  • Glycosyltransferases / metabolism*
  • Molecular Conformation
  • Molecular Probes / chemistry
  • Molecular Probes / metabolism*

Substances

  • Diterpenes, Kaurane
  • Molecular Probes
  • steviol
  • Glycosyltransferases