Antibiotic Susceptibility Pattern of Gram-negative Isolates of Lower Respiratory Tract Infection

J Nepal Health Res Counc. 2018 Mar 13;16(1):22-26.

Abstract

Background: Lower respiratory tract infection is a common cause of morbidity and mortality worldwide. A cross-sectional study was carried out with an objective to study the antibiogram of Gram-negative isolates of patients with lower respiratory tract infection visiting Kathmandu Model Hospital.

Methods: A total of 274 specimens including sputum, endotracheal aspirates, suction tips were cultured as per standard microbiological technique. Antibiotic susceptibility and detection of Extended-spectrum beta- lactamases (ESBLs) were performed following Clinical Laboratory Standard Institute (CLSI 2014) guidelines.

Results: Respiratory pathogens were recovered from 24.6% (n=65) cases. Klebsiella pneumoniae (40%) was the commonest isolates. The highest prevalence of multidrug-resistance (69.23%) was observed in Acinetobacter calcoaceticus baumannii complex. Extended-spectrum beta- lactamases were detected in Escherichia coli (n=4), Klebsiella pneumoniae (n=4) and Acinetobacter calcoaceticus baumannii complex (n=1).

Conclusions: High prevalence of multidrug-resistance and extended- spectrum beta- lactamase producers were observed in respiratory isolates. For effective management of lower respiratory tract infections, an ultimate and detailed microbiological diagnosis and susceptibility testing is required.

MeSH terms

  • Cross-Sectional Studies
  • Drug Resistance, Multiple, Bacterial*
  • Escherichia coli / drug effects*
  • Humans
  • Klebsiella / drug effects*
  • Klebsiella Infections / drug therapy
  • Nepal
  • Respiratory Tract Infections / drug therapy*
  • Respiratory Tract Infections / physiopathology
  • Tertiary Care Centers