Stable isotopes of hydrogen and oxygen (δ2H, δ18O and δ17O) can be used as natural tracers to improve our understanding of hydrological and meteorological processes. Studies of precipitation isotopes, especially 17O-excess observations, are extremely limited in the mid-latitudes. To fill this knowledge gap, we measured δ2H, δ18O and δ17O of event-based precipitation samples collected from Indianapolis, Indiana, USA over two years and investigated the influence of meteorological factors on precipitation isotope variations. The results showed that the daily temperature played a major role in controlling the isotope variations. Precipitation experienced kinetic fractionation associated with evaporation at the moisture source in the spring and summer and for rainfall, while snowfall, as well as precipitation in the fall and winter, were mainly affected by equilibrium fractionation. The 17O-excess of both rainfall and snowfall were not affected by local meteorological factors over the whole study period. At the seasonal scale, it was the case only for the spring. Therefore, 17O-excess of rainfall, snowfall and the spring precipitation could be considered as tracers of evaporative conditions at the moisture source. This study provides a unique precipitation isotope dataset for mid-latitudes and provides a more mechanistic understanding of precipitation formation mechanisms in this region.