Macrophage Migration Inhibitory Factor -173GC Variant Might Increase the Risk of Behçet's Disease

Med Princ Pract. 2018;27(3):285-289. doi: 10.1159/000489340. Epub 2018 Apr 18.

Abstract

Objective: The aim of the present study was to investigate any possible association between the macrophage migration inhibitory factor (MIF) -173GC variant and Behçet's disease (BD) in a group of Turkish patients.

Subjects and methods: A total of 111 patients with BD and 100 healthy controls were enrolled in this study. Genomic DNA was extracted from peripheral lymphocytes. The MIF -173GC variant was genotyped using polymerase chain reaction restriction fragment length polymorphism. The allele and genotype frequencies of patients and controls were compared using the χ2 test.

Results: A statistically significant difference in the distribution of the genotype was observed between BD patients and healthy controls. The homo-genotype CC was more prevalent in the patient group compared to the control group (p = 0.008, OR: 0.24, 95% Cl: 0.05-0.78). A significant association was observed when the patients were compared with the controls according to GG + GC versus CC ge-notypes (p = 0.003, OR: 1.21, 95% CI: 0.06-0.063). Allele frequencies of the MIF -173GC variant did not show any statistically significant difference between patients and controls.

Conclusion: In this study, we conclude that the CC ge-notype of the MIF -173GC variant may be a risk factor in the pathogenesis of BD in the Turkish population. However, further studies with larger samples are needed to address the exact role of this variant in BD.

Keywords: Behçet’s disease; Macrophage migration inhibitory factor; Variant.

MeSH terms

  • Behcet Syndrome / genetics*
  • Case-Control Studies
  • Female
  • Gene Frequency
  • Genotype*
  • Genotyping Techniques
  • Humans
  • Intramolecular Oxidoreductases / genetics*
  • Macrophage Migration-Inhibitory Factors / genetics*
  • Male
  • Polymorphism, Restriction Fragment Length*
  • RNA, Messenger / genetics
  • Turkey

Substances

  • Macrophage Migration-Inhibitory Factors
  • RNA, Messenger
  • Intramolecular Oxidoreductases
  • MIF protein, human