Exome sequencing reveals a novel PLP1 mutation in a Moroccan family with connatal Pelizaeus-Merzbacher disease: a case report

BMC Pediatr. 2018 Feb 27;18(1):90. doi: 10.1186/s12887-018-1063-5.

Abstract

Background: Epilepsy regroups a common and diverse set of chronic neurological disorders that are characterized by spontaneous, unprovoked, and recurrent epileptic seizures. Epilepsies have a highly heterogeneous background with a strong genetic contribution and various mode of inheritance. X-linked epilepsy usually manifests as part of a syndrome or epileptic encephalopathy. The variability of clinical manifestations of X-linked epilepsy may be attributed to several factors including the causal genetic mutation, making diagnosis, genetic counseling and treatment decisions difficult. We report the description of a Moroccan family referred to our genetic department with X-linked epileptic seizures as the only initial diagnosis.

Case presentation: Knowing the new contribution of Next-Generation Sequencing (NGS) for clinical investigation, and given the heterogeneity of this group of disorders we performed a Whole-Exome Sequencing (WES) analysis and co-segregation study in several members of this large family. We detected a novel pathogenic PLP1 missense mutation c.251C > A (p.Ala84Asp) allowing us to make a diagnosis of Pelizaeus-Merzbacher Disease for this family.

Conclusion: This report extends the spectrum of PLP1 mutations and highlights the diagnostic utility of NGS to investigate this group of heterogeneous disorders.

Keywords: Connatal Pelizaeus-Merzbacher disease; Missense mutation; PLP1 gene.

Publication types

  • Case Reports

MeSH terms

  • Child, Preschool
  • Exome Sequencing*
  • Family
  • Female
  • Genetic Markers
  • Humans
  • Male
  • Morocco
  • Mutation, Missense*
  • Myelin Proteolipid Protein / genetics*
  • Pedigree
  • Pelizaeus-Merzbacher Disease / diagnosis*
  • Pelizaeus-Merzbacher Disease / genetics*

Substances

  • Genetic Markers
  • Myelin Proteolipid Protein
  • PLP1 protein, human