De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing

Jessica M Bryant; Sebastian Baumgarten; Audrey Lorthiois; Christine Scheidig-Benatar; Aurélie Claës; Artur Scherf

doi:10.1128/genomeA.01479-17

De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing

Genome Announc. 2018 Feb 1;6(5):e01479-17. doi: 10.1128/genomeA.01479-17.

Authors

Jessica M Bryant^{1

2

3}, Sebastian Baumgarten^{4

2

3}, Audrey Lorthiois⁵, Christine Scheidig-Benatar^{4

2

3}, Aurélie Claës^{4

2

3}, Artur Scherf^{1

2

3}

Affiliations

¹ Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France jessica.bryant@pasteur.fr artur.scherf@pasteur.fr.
² INSERM U1201, Paris, France.
³ CNRS ERL9195, Paris, France.
⁴ Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France.
⁵ Center for Production and Infection of Anopheles (CEPIA), Institut Pasteur, Paris, France.

Abstract

Plasmodium falciparum is the species of human malaria parasite that causes the most severe form of the disease. Here, we used single-molecule real-time (SMRT) sequencing technology from Pacific Biosciences (PacBio) to sequence, assemble de novo, and annotate the genome of a P. falciparum NF54 clone.