The DNA methylation status of genes encoding Matrix metalloproteinases and tissue inhibitors of Matrix metalloproteinases in endometriosis

Mol Reprod Dev. 2018 Jan;85(1):17-25. doi: 10.1002/mrd.22931. Epub 2017 Dec 21.

Abstract

Endometriosis is a benign disease, with malignant properties. A necessary step in the progression of endometriosis is tissue remodeling, which is coordinated by the activities of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs). This study evaluated the regulation of abnormal MMP and TIMP gene expression during endometriosis. Among the two genes families, promoter regions of MMP2, MMP3, MMP7, TIMP3, and TIMP4 were significantly altered in proliferative-phase endometriotic lesions compared to menstrual cycle-matched eutopic tissue from endometriosis-free women. In addition, a negative correlation was found between the DNA methylation status of the promoter region and transcript abundance of MMP2. Our findings suggest that changes in DNA methylation at the promoter region of MMP2 could underlie the changes in its expression in the ectopic endometria from patients with endometriosis.

Keywords: correlation; gene expression; promoter region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Methylation / genetics*
  • Endometriosis / genetics*
  • Endometriosis / pathology
  • Female
  • Gene Expression Regulation / genetics
  • Humans
  • Matrix Metalloproteinase 2 / genetics*
  • Matrix Metalloproteinase 3 / genetics
  • Matrix Metalloproteinase 7 / genetics
  • Menstrual Cycle / physiology
  • Promoter Regions, Genetic / genetics*
  • Tissue Inhibitor of Metalloproteinase-3 / genetics
  • Tissue Inhibitor of Metalloproteinase-4
  • Tissue Inhibitor of Metalloproteinases / genetics

Substances

  • TIMP3 protein, human
  • Tissue Inhibitor of Metalloproteinase-3
  • Tissue Inhibitor of Metalloproteinases
  • MMP3 protein, human
  • Matrix Metalloproteinase 3
  • MMP7 protein, human
  • Matrix Metalloproteinase 7
  • MMP2 protein, human
  • Matrix Metalloproteinase 2