Antimicrobial and Antibiofilm Activity of Human Milk Oligosaccharides against Streptococcus agalactiae, Staphylococcus aureus, and Acinetobacter baumannii

Dorothy L Ackerman; Kelly M Craft; Ryan S Doster; Jörn-Hendrik Weitkamp; David M Aronoff; Jennifer A Gaddy; Steven D Townsend

doi:10.1021/acsinfecdis.7b00183

Antimicrobial and Antibiofilm Activity of Human Milk Oligosaccharides against Streptococcus agalactiae, Staphylococcus aureus, and Acinetobacter baumannii

ACS Infect Dis. 2018 Mar 9;4(3):315-324. doi: 10.1021/acsinfecdis.7b00183. Epub 2017 Dec 8.

Authors

Dorothy L Ackerman¹, Kelly M Craft¹, Ryan S Doster², Jörn-Hendrik Weitkamp^{2

3}, David M Aronoff², Jennifer A Gaddy^{2

4}, Steven D Townsend^{1

5}

Affiliations

¹ Department of Chemistry , Vanderbilt University , 7330 Stevenson Center , Nashville , Tennessee 37235 , United States.
² Department of Medicine , Vanderbilt University Medical Center , 1161 21st Avenue South, D-3100 Medical Center North , Nashville , Tennessee 37232 , United States.
³ Department of Pediatrics , Monroe Carell Jr. Children's Hospital at Vanderbilt , 2200 Children's Way, Suite 2404 , Nashville , Tennessee 37232 , United States.
⁴ Tennessee Valley Healthcare Systems , Department of Veterans Affairs , 1310 24th Avenue South , Nashville , Tennessee 37212 , United States.
⁵ Institute of Chemical Biology , Vanderbilt University , 896 Preston Research Building , Nashville , Tennessee 37232-6304 , United States.

Abstract

In a previous study, we reported that human milk oligosaccharides (HMOs) isolated from five donor milk samples possessed antimicrobial and antibiofilm activity against Streptococcus agalactiae, also known as Group B Streptococcus or GBS. Herein, we present a broader evaluation of the antimicrobial and antibiofilm activity by screening HMOs from 14 new donors against three strains of GBS and two of the ESKAPE pathogens of particular interest to child health, Staphylococcus aureus and Acinetobacter baumannii. Growth and biofilm assays showed that HMOs from these new donors possessed antimicrobial and antibiofilm activity against all three strains of GBS, antibiofilm activity against methicillin-resistant S. aureus strain USA300, and antimicrobial activity against A. baumannii strain ATCC 19606.

Keywords: A. baumannii; GBS; S. agalactiae; S. aureus; antibiofilm; antimicrobial.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / growth & development
Anti-Bacterial Agents / isolation & purification
Anti-Bacterial Agents / pharmacology*
Biofilms / drug effects*
Biofilms / growth & development
Biological Products / isolation & purification
Biological Products / pharmacology
Female
Humans
Microbial Sensitivity Tests
Milk, Human / chemistry*
Oligosaccharides / isolation & purification
Oligosaccharides / pharmacology*
Staphylococcus aureus / drug effects*
Staphylococcus aureus / growth & development
Streptococcus agalactiae / drug effects*
Streptococcus agalactiae / growth & development

Substances

Anti-Bacterial Agents
Biological Products
Oligosaccharides

Abstract

Publication types

MeSH terms

Substances

Grants and funding