RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain

Nat Commun. 2017 Nov 2;8(1):1271. doi: 10.1038/s41467-017-01333-6.

Abstract

Although ubiquitin ligases have been implicated in autism, their roles and mechanisms in brain development remain incompletely understood. Here, we report that in vivo knockdown or conditional knockout of the autism-linked ubiquitin ligase RNF8 or associated ubiquitin-conjugating enzyme UBC13 in rodent cerebellar granule neurons robustly increases the number of parallel fiber presynaptic boutons and functional parallel fiber/Purkinje cell synapses. In contrast to the role of nuclear RNF8 in proliferating cells, RNF8 operates in the cytoplasm in neurons to suppress synapse differentiation in vivo. Proteomics analyses reveal that neuronal RNF8 interacts with the HECT domain protein HERC2 and scaffold protein NEURL4, and knockdown of HERC2 or NEURL4 phenocopies the inhibition of RNF8/UBC13 signaling on synapse differentiation. In behavior analyses, granule neuron-specific knockout of RNF8 or UBC13 impairs cerebellar-dependent learning. Our study defines RNF8 and UBC13 as components of a novel cytoplasmic ubiquitin-signaling network that suppresses synapse formation in the brain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / growth & development*
  • Cerebellum / cytology
  • Gene Knockdown Techniques
  • Gene Knockout Techniques
  • Guanine Nucleotide Exchange Factors / metabolism
  • Mice
  • Microscopy, Electron
  • Neurons / metabolism*
  • Patch-Clamp Techniques
  • Presynaptic Terminals / metabolism
  • Presynaptic Terminals / ultrastructure
  • Proteomics
  • Purkinje Cells / metabolism
  • Purkinje Cells / ultrastructure
  • Rats
  • Signal Transduction
  • Synapses / metabolism
  • Synapses / ultrastructure*
  • Ubiquitin-Conjugating Enzymes / genetics*
  • Ubiquitin-Protein Ligases / genetics*

Substances

  • Guanine Nucleotide Exchange Factors
  • Herc2 protein, mouse
  • Ube2n protein, mouse
  • Ube2n protein, rat
  • Ubiquitin-Conjugating Enzymes
  • Rnf8 protein, mouse
  • Rnf8 protein, rat
  • Ubiquitin-Protein Ligases