Whole-Body Biodistribution, Dosimetry, and Metabolite Correction of [11C]Palmitate: A PET Tracer for Imaging of Fatty Acid Metabolism

Mol Imaging. 2017 Jan-Dec:16:1536012117734485. doi: 10.1177/1536012117734485.

Abstract

Introduction: Despite the decades long use of [11C]palmitate positron emission tomography (PET)/computed tomography in basic metabolism studies, only personal communications regarding dosimetry and biodistribution data have been published.

Methods: Dosimetry and biodistribution studies were performed in 2 pigs and 2 healthy volunteers by whole-body [11C]palmitate PET scans. Metabolite studies were performed in 40 participants (healthy and with type 2 diabetes) under basal and hyperinsulinemic conditions. Metabolites were estimated using 2 approaches and subsequently compared: Indirect [11C]CO2 release and parent [11C]palmitate measured by a solid-phase extraction (SPE) method. Finally, myocardial fatty acid uptake was calculated in a patient cohort using input functions derived from individual metabolite correction compared with population-based metabolite correction.

Results: In humans, mean effective dose was 3.23 (0.02) µSv/MBq, with the liver and myocardium receiving the highest absorbed doses. Metabolite correction using only [11C]CO2 estimates underestimated the fraction of metabolites in studies lasting more than 20 minutes. Population-based metabolite correction showed excellent correlation with individual metabolite correction in the cardiac PET validation cohort.

Conclusion: First, mean effective dose of [11C]palmitate is 3.23 (0.02) µSv/MBq in humans allowing multiple scans using ∼300 MBq [11C]palmitate, and secondly, population-based metabolite correction compares well with individual correction.

Keywords: PET/CT; advances in PET/SPECT probes; cardiac imaging; metabolism; quantitation in molecular imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon Radioisotopes / metabolism*
  • Female
  • Humans
  • Image Processing, Computer-Assisted
  • Kinetics
  • Male
  • Metabolome*
  • Middle Aged
  • Palmitates / metabolism*
  • Positron-Emission Tomography*
  • Radiometry*
  • Radiopharmaceuticals / chemistry*
  • Solid Phase Extraction
  • Sus scrofa
  • Tissue Distribution

Substances

  • Carbon Radioisotopes
  • Carbon-11
  • Palmitates
  • Radiopharmaceuticals