Objective: To investigate the relationship between serum/follicular fluid fetuin B levels and outcome of in vitro fertilization (IVF).
Methods: Infertility women (28 with low fertilization rates, 44 with normal fertilization rates) receiving IVF in Women's Hospital of Zhejiang University School of Medicine during June and December 2016 were enrolled in the study. Serum/follicular fluid fetuin B levels were measured with ELISA method. Correlations of serum and follicular fetuin B level with fertilization outcome of IVF were analyzed with Pearson correlation coefficient and receiver operating characteristic (ROC) curve.
Results: A positive correlation between serum fetuin B and follicular fluid fetuin B levels was observed (r=0.675, P<0.01). Both serum and follicular fluid fetuin B levels in women with low fertilization rates of IVF were lower than those in women with normal fertilization rates[(6.09±1.31) μg/mL vs. (7.13±1.47) μg/mL, t=3.050, P<0.05; (5.13±0.96)μg/mL vs. (6.22±1.33) μg/mL, t=3.755, P<0.01]. ROC analysis showed that the area under curve (AUC) of serum fetuin B level in predicting fertilization rate was 0.832 (95% CI:0.729-0.934, P<0.01), and 6.08 μg/mL could be used as cut-off value.
Conclusions: Serum fetuin B level is correlated with follicular fluid fetuin B level, and it may be used for predicting the fertilization outcome of IVF.
目的: 探究血清和卵泡液中胎球蛋白B水平与体外受精结局的相关性。
方法: 收集2016年6-12月在浙江大学医学院附属妇产科医院行体外受精的女性患者（受精低下组28个周期，受精正常组44个周期）。利用ELISA法检测血清和卵泡液中胎球蛋白B的水平。采用Pearson相关性分析和受试者工作特征曲线等方法分析血清和卵泡液中胎球蛋白B水平与受精结局的关系。
结果: 血清中胎球蛋白B水平与卵泡液中胎球蛋白B的水平呈正相关（ r=0.675， P < 0.01）。受精低下组与受精正常组血清中胎球蛋白B的水平分别为（6.09±1.31）μg/mL和（7.13±1.47）μg/mL，差异有统计学意义（ t=3.050， P < 0.05）；两组卵泡液中胎球蛋白B的水平分别为（5.13±0.96）μg/mL和（6.22±1.33）μg/mL，差异有统计学意义（ t=3.755， P < 0.01）。当血清中胎球蛋白B的水平低于6.08 μg/mL时，受精率下降（AUC=0.832，95% CI：0.729~0.934， P < 0.01）。
结论: 血清中胎球蛋白B的水平可以反映卵泡液中胎球蛋白B的水平，并可在一定程度上预测受精结局。