Hox genes reveal genomic DNA variation in tetraploid hybrids derived from Carassius auratus red var. (female) × Megalobrama amblycephala (male)

Y D Wang; Q B Qin; R Yang; W Z Sun; Q W Liu; Y Y Huo; X Huang; M Tao; C Zhang; T Li; S J Liu

doi:10.1186/s12863-017-0550-2

Hox genes reveal genomic DNA variation in tetraploid hybrids derived from Carassius auratus red var. (female) × Megalobrama amblycephala (male)

BMC Genet. 2017 Oct 11;18(1):86. doi: 10.1186/s12863-017-0550-2.

Authors

Y D Wang^{1

2}, Q B Qin^{1

2}, R Yang^{1

2}, W Z Sun^{1

2}, Q W Liu^{1

2}, Y Y Huo^{1

2}, X Huang^{1

2}, M Tao^{1

2}, C Zhang^{1

2}, T Li^{1

2}, S J Liu^{3

4}

Affiliations

¹ State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China.
² College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China.
³ State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China. lsj@hunnu.edu.cn.
⁴ College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China. lsj@hunnu.edu.cn.

Abstract

Background: Allotetraploid F₁ hybrids (4nF₁) (AABB, 4n = 148) were generated from the distant hybridization of Carassius auratus red var. (RCC) (AA, 2n = 100) (♀) × Megalobrama amblycephala (BSB) (BB, 2n = 48) (♂). It has been reported that Hox gene clusters are highly conserved among plants and vertebrates. In this study, we investigated the genomic organization of Hox gene clusters in the allotetraploid F₁ hybrids and their parents to investigate the polyploidization process.

Results: There were three copies of Hox genes in the 4nF₁ hybrids, two copies in RCC and one copy in BSB. In addition, obvious variation and pseudogenization were observed in some Hox genes from 4nF_1. CONCLUSION: Our results reveal the influence of polyploidization on the organization and evolution of Hox gene clusters in fish and also clarify some aspects of vertebrate genome evolution.

Keywords: Allotetraploid; Hox gene; polyploidization; pseudogenization.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Genes, Homeobox / physiology*
Genetic Variation*
Goldfish / classification
Goldfish / genetics*
Hybridization, Genetic
Karyotyping
Male
Phylogeny
Sequence Analysis, DNA / methods
Tetraploidy*