Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors

Raymond C B Wong; Sandy S Hung; Stacey Jackson; Vikrant Singh; Shahnaz Khan; Helena H Liang; Lisa S Kearns; Tu Nguyen; Alison Conquest; Maciej Daniszewski; Alex W Hewitt; Alice Pébay

doi:10.1016/j.scr.2017.05.007

Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors

Stem Cell Res. 2017 Jul:22:13-15. doi: 10.1016/j.scr.2017.05.007. Epub 2017 May 19.

Authors

Affiliations

¹ Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Australia; Ophthalmology, Department of Surgery, The University of Melbourne, Australia. Electronic address: wongcb@unimelb.edu.au.
² Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Australia; Ophthalmology, Department of Surgery, The University of Melbourne, Australia.
³ School of Medicine, Menzies Institute for Medical Research, University of Tasmania, Australia.
⁴ Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Australia; Ophthalmology, Department of Surgery, The University of Melbourne, Australia; School of Medicine, Menzies Institute for Medical Research, University of Tasmania, Australia.
⁵ Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Australia; Ophthalmology, Department of Surgery, The University of Melbourne, Australia. Electronic address: apebay@unimelb.edu.au.

PMID: 28952926
DOI: 10.1016/j.scr.2017.05.007

Abstract

We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.

MeSH terms

Cell Line
Fibroblasts / cytology
Humans
Immunohistochemistry
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Kruppel-Like Factor 4
Male
Middle Aged
Plasmids / genetics
Plasmids / metabolism*