Growing Chlorella vulgaris on thermophilic anaerobic digestion swine manure for nutrient removal and biomass production

Xiang-Yuan Deng; Kun Gao; Ren-Chuan Zhang; Min Addy; Qian Lu; Hong-Yan Ren; Paul Chen; Yu-Huan Liu; Roger Ruan

doi:10.1016/j.biortech.2017.06.141

Growing Chlorella vulgaris on thermophilic anaerobic digestion swine manure for nutrient removal and biomass production

Bioresour Technol. 2017 Nov:243:417-425. doi: 10.1016/j.biortech.2017.06.141. Epub 2017 Jun 26.

Authors

Xiang-Yuan Deng¹, Kun Gao¹, Ren-Chuan Zhang², Min Addy², Qian Lu², Hong-Yan Ren³, Paul Chen², Yu-Huan Liu⁴, Roger Ruan⁵

Affiliations

¹ College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, People's Republic of China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States.
² Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States.
³ Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States; School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, People's Republic of China.
⁴ The Engineering Research Center for Biomass Conversion, Ministry of Education, People's Republic of China, Nanchang University, Nanchang 330047, People's Republic of China.
⁵ Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States; The Engineering Research Center for Biomass Conversion, Ministry of Education, People's Republic of China, Nanchang University, Nanchang 330047, People's Republic of China. Electronic address: ruanx001@umn.edu.

PMID: 28688324
DOI: 10.1016/j.biortech.2017.06.141

Abstract

Liquid swine manure was subjected to thermophilic anaerobic digestion, ammonia stripping and centrifugation in order to increase the available carbon sources and decrease the ammonia concentration and turbidity. Chlorella vulgaris (UTEX 2714) was grown on minimally diluted (2×, 3× and 4×) autoclaved and non-autoclaved pretreated anaerobic digestion swine manure (PADSM) in a batch-culture system for 7days. Results showed that C. vulgaris (UTEX 2714) grew best on 3× PADSM media, and effectively removed NH₄⁺-N, TN, TP and COD by 98.5-99.8%, 49.2-55.4%, 20.0-29.7%, 31.2-34.0% and 99.8-99.9%, 67.4-70.8%, 49.3-54.4%, 73.6-78.7% in differently diluted autoclaved and non-autoclaved PADSM, respectively. Results of chemical compositions indicated that contents of pigment, carbohydrate, protein and lipid in C. vulgaris (UTEX 2714) changed with the culture conditions. Moreover, its fatty acid profiles suggested that this alga could be used as animal feed if cultivated in autoclaved PADSM or as good-quality biodiesel feedstock if cultivated in non-autoclaved PADSM.

Keywords: Chemical composition; Chlorella vulgaris; Fatty acid profiles; Nutrient removal; Swine manure.

MeSH terms

Anaerobiosis
Animals
Biofuels*
Biomass
Chlorella vulgaris*
Manure*
Swine

Substances

Biofuels
Manure