Generation of a Sprague-Dawley-GFP rat iPS cell line

Giulia Coppiello; Gloria Abizanda; Natalia Aguado; Elena Iglesias; Olalla Iglesias-Garcia; Antonio Lo Nigro; Felipe Prosper; Xabier L Aranguren

doi:10.1016/j.scr.2017.03.021

Generation of a Sprague-Dawley-GFP rat iPS cell line

Stem Cell Res. 2017 May:21:47-50. doi: 10.1016/j.scr.2017.03.021. Epub 2017 Mar 31.

Authors

Giulia Coppiello¹, Gloria Abizanda¹, Natalia Aguado¹, Elena Iglesias¹, Olalla Iglesias-Garcia², Antonio Lo Nigro³, Felipe Prosper⁴, Xabier L Aranguren⁵

Affiliations

¹ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
² Center of Regenerative Medicine in Barcelona (CMRB), Spain.
³ Ri.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, Italy.
⁴ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: fprosper@unav.es.
⁵ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: xlaranguren@unav.es.

PMID: 28677538
DOI: 10.1016/j.scr.2017.03.021

Abstract

We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Green Fluorescent Proteins / biosynthesis*
Green Fluorescent Proteins / genetics
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Rats
Rats, Transgenic

Substances

Green Fluorescent Proteins