Research involving biosafety level 3 pathogens such as West Nile virus (WNV) is often limited by the limited space and technical constraints of these environments. To conduct complex analytical studies outside of high containment, robust and reliable inactivation methods are needed that maintain compatibility with downstream assays. Here we report the inactivation of WNV in spiked serum samples using a commercially available SDS-PAGE sample buffer for proteomic studies. Using this method, we demonstrate its utility by identification proteins differentially expressed in the serum of mice experimentally infected with WNV.
Keywords: BSL-3; Dithiothreitol; Lithium dodecyl sulfate; Sample buffer; Virus inactivation; West Nile virus.
Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.